Septins are members of a conserved family of cytoskeletal GTPases present in organisms as
diverse as yeast and mammals. Unlike lower eukaryotic cells, the physiological significance
of mammalian septin complexes is largely unknown. Using specific antibodies, we found at
least five septins, Sept2, Sept7, Sept8, Sept9b, and Sept11, in septin complexes affinity-
purified with anti-Sept7 antibody-conjugated column from rat embryonic fibroblast REF52
cells. Immunofluorescence studies revealed co-localization of Sept7, Sept9b, and Sept11 …