Tissue damage by oxidative stress is a key pathogenic mechanism in various diseases,
including AKI and CKD. Thus, early detection of oxidative tissue damage is important. Using
a tRNA-specific modified nucleoside 1-methyladenosine (m1A) antibody, we show that
oxidative stress induces a direct conformational change in tRNA structure that promotes
subsequent tRNA fragmentation and occurs much earlier than DNA damage. In various
models of tissue damage (ischemic reperfusion, toxic injury, and irradiation), the levels of …