DNA fragment sizing and sorting by laser-induced fluorescence

JH Jett, ML Hammond, RA Keller, BL Marrone… - 1992 - osti.gov
… be bound to the DNA piece and DNA fragments to provide … DNA fragments to provide
information on oligonucleotide distribution or probe hybridization to DNA fragments of different sizes

Single-molecule detection in liquids by laser-induced fluorescence

PM Goodwin, WP Ambrose… - Accounts of Chemical …, 1996 - ACS Publications
… burst rates are consistently at least an order of magnitude higher than from ultrapure water.
… We have used this method to size DNA fragments between 500 and 167 000 bp in length. …

Sizing of DNA fragments by flow cytometry

ME Johnson, PM Goodwin… - Ultrasensitive …, 1993 - spiedigitallibrary.org
sorting capabilities of flow cytometry to sort individual DNA fragments according to size, in
order … single molecules in flowing sample streams by laser-induced fluorescence," Proc. SPIE, …

[HTML][HTML] Quantifying double-strand breaks and clustered damages in DNA by single-molecule laser fluorescence sizing

EM Filippova, DC Monteleone, JG Trunk… - Biophysical journal, 2003 - cell.com
… flash of fluorescence is recorded and used to determine the size of … laser-induced flashes
are a linear function of the size of the DNA and demonstrate that molecules of different sizes

Flow cytometric sizing of DNA fragments

WP Ambrose, H Cai, PM Goodwin, JH Jett… - Topics in Fluorescence …, 2003 - Springer
… and either a fourth order polynomial or a decaying exponential … the fit and plotted against
the known DNA fragment sizes. … in liquids by laserinduced fluorescence. Accounts Chem. Res. …

Application of single molecule detection to DNA sequencing and sizing

WP Ambrose, PM Goodwin, JH Jett… - Berichte der …, 1993 - Wiley Online Library
DNA fragment to a support, movement of the supported DNA … by laser-induced fluorescence
as they are cleaved from the … We also plan to use the sorting capabilities of flow cytometry …

Effects of fluorescence excitation geometry on the accuracy of DNA fragment sizing by flow cytometry

JH Werner, EJ Larson, PM Goodwin, WP Ambrose… - Applied Optics, 2000 - opg.optica.org
… burst size and DNA fragment length has been established in our apparatus for a wide range
of DNA fragment sizes (0.3–… The laser-induced fluorescence generated by the stained DNA

Exploiting sensitive laserinduced fluorescence detection on electrophoretic microchips for executing rapid clinical diagnostics

J Ferrance, JP Landers - … : the journal of biological and chemical …, 2001 - Wiley Online Library
size-based separation of the DNA fragments on a slab gel, which is then stained with ethidium
bromide to allow for visualization of the DNA by fluorescence. This … In order to achieve …

[图书][B] Revolutionizing genomic analyses: mutation analyses using novel enzyme-based assays with laser-induced fluorescence and polymeric microfluidic devices as …

RDS Sinville - 2008 - search.proquest.com
… An illustration of the use of a porous gel medium to electrophoretically sort a mixed DNA
population into bands in … the pore size of a gel medium relative to the DNA size distribution. For …

Multi-color fluorescent DNA analysis in an integrated optofluidic lab on a chip

C Dongre - 2010 - research.utwente.nl
Sorting and sizing of DNA molecules within the human genome … laser-induced fluorescence
for optofluidic integration toward an on-chip bio-analysis tool. Integrated optical fluorescence