Detection of bacterial pathogens in municipal wastewater using an oligonucleotide microarray and real-time quantitative PCR
DY Lee, K Shannon, LA Beaudette - Journal of microbiological methods, 2006 - Elsevier
As a first step toward building a comprehensive microarray, two low density DNA
microarrays were constructed and evaluated for the accurate detection of wastewater
pathogens. The first one involved the direct hybridization of wastewater microbial genomic
DNA to the functional gene probes while the second involved PCR amplification of 23S
ribosomal DNA. The genomic DNA microarray employed 10 functional genes as detection
targets. Sensitivity of the microarray was determined to be approximately 1.0 μg of …
microarrays were constructed and evaluated for the accurate detection of wastewater
pathogens. The first one involved the direct hybridization of wastewater microbial genomic
DNA to the functional gene probes while the second involved PCR amplification of 23S
ribosomal DNA. The genomic DNA microarray employed 10 functional genes as detection
targets. Sensitivity of the microarray was determined to be approximately 1.0 μg of …
Detection of bacterial pathogens in municipal wastewater using an oligonucleotide microarray and real-time quantitative PCR.
LDY Lee DaeYoung, K Shannon, LA Beaudette - 2006 - cabidigitallibrary.org
As a first step toward building a comprehensive microarray, two low density DNA
microarrays were constructed and evaluated for the accurate detection of wastewater
pathogens. The first one involved the direct hybridization of wastewater microbial genomic
DNA to the functional gene probes while the second involved PCR amplification of 23S
ribosomal DNA. The genomic DNA microarray employed 10 functional genes as detection
targets. Sensitivity of the microarray was determined to be approximately 1.0 µg of …
microarrays were constructed and evaluated for the accurate detection of wastewater
pathogens. The first one involved the direct hybridization of wastewater microbial genomic
DNA to the functional gene probes while the second involved PCR amplification of 23S
ribosomal DNA. The genomic DNA microarray employed 10 functional genes as detection
targets. Sensitivity of the microarray was determined to be approximately 1.0 µg of …
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