Effect of growth hormone and insulin-like growth factor-I on spontaneous apoptosis in cultured luteal cells collected from early, mature, and regressing porcine corpora …
A Ptak, M Kajta, EL Gregoraszczuk - Animal reproduction science, 2004 - Elsevier
The aim of the present study was to test the hypothesis that growth hormone (GH) and
insulin-like growth factor-I (IGF-I) act at a local level to inhibit luteal cell apoptosis. Luteal
cells collected from the corpora lutea at different stages of the luteal phase were cultured for
24h in M 199 medium supplemented with 5% of calf serum to cause attachment cells to the
plastic. After 24h, the media were changed and various concentrations of GH (10, 100 or
200ng/ml) or IGF-I (30, 50 or 100ng/ml) were added to the culture medium. Twenty-four …
insulin-like growth factor-I (IGF-I) act at a local level to inhibit luteal cell apoptosis. Luteal
cells collected from the corpora lutea at different stages of the luteal phase were cultured for
24h in M 199 medium supplemented with 5% of calf serum to cause attachment cells to the
plastic. After 24h, the media were changed and various concentrations of GH (10, 100 or
200ng/ml) or IGF-I (30, 50 or 100ng/ml) were added to the culture medium. Twenty-four …
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