[HTML][HTML] High heterogeneity of fecal carriage extended-spectrum beta-lactamase-producing E. coli isolated from iranian community and clinical settings
S Aghamohammad, VS Nikbin, F Badmasti… - BMC Infectious …, 2022 - Springer
S Aghamohammad, VS Nikbin, F Badmasti, F Shahcheraghi
BMC Infectious Diseases, 2022•SpringerBackground Extended-spectrum beta-lactamase-producing enterobacteria (ESBL-PE) in
carriers have become a global health problem. Using molecular typing techniques, including
PFGE, could be useful to determine the source of bacterial dissemination. The current study
aimed to investigate the intestinal carriage of ESBL-producing E. coli (ESBL-EC) and clonal
relatedness among ESBL-EC isolated from hospitalized and outpatient fecal carriers in Iran.
Methods A total of 120 rectal swabs were collected; 50.8%(61/120) from intensive care unit …
carriers have become a global health problem. Using molecular typing techniques, including
PFGE, could be useful to determine the source of bacterial dissemination. The current study
aimed to investigate the intestinal carriage of ESBL-producing E. coli (ESBL-EC) and clonal
relatedness among ESBL-EC isolated from hospitalized and outpatient fecal carriers in Iran.
Methods A total of 120 rectal swabs were collected; 50.8%(61/120) from intensive care unit …
Background
Extended-spectrum beta-lactamase-producing enterobacteria (ESBL-PE) in carriers have become a global health problem. Using molecular typing techniques, including PFGE, could be useful to determine the source of bacterial dissemination. The current study aimed to investigate the intestinal carriage of ESBL-producing E. coli (ESBL-EC) and clonal relatedness among ESBL-EC isolated from hospitalized and outpatient fecal carriers in Iran.
Methods
A total of 120 rectal swabs were collected; 50.8% (61/120) from intensive care unit (ICU) inpatients and 49.2% (59/120) from outpatients. MacConkey agar enriched with cefotaxime was used to screen the ESBL-EC. PCR assays were performed to detect ESBL and carbapenemase genes. Pulse-fields gel electrophoresis (PFGE) was performed to assess clonal relatedness.
Results
Totally, 60.0% (72/120) were carrier for ESBL-EC. The rates of resistance against ceftazidime and cefepime were 90.2% (65/72) and 93.0% (67/72), respectively. The rates of blaCTX-M-15, blaTEM, blaSHV, blaNDM-1, blaOXA-48 and blaIMP was 90.2% (65/72), 50.0% (36/72), 5.5% (4/72), 4.1% (3/72), 4.1% (3/72) and 1.3% (1/72), respectively. Based on a cut-off 80%, 69 ESBL-EC isolates could be categorized in 10 mini-cluster and 47 isolates were considered as singletons.
Discussion
High heterogeneity among isolates from ESBL-EC suggests that this bacterium probably has a different source of dissemination. Screening of carriers in hospitals and communities could help the infection control program in public health.
Springer
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