The pytochemicals, vitamins and mineral constituents of ethanol leaves extract of Piper nigrum was investigated in this study. The preliminary phytochemical screening showed that ethanol leaves extract of Piper nigrum contains alkaloids, flavonoids, steroids, tannins, saponins and terpenoids while cardiac glycosides were not dictated. The quantitative phytochemical analysis contains: alkaloids (17.27±0.05 mg/g), steroids (6.06±0.00 mg/g), terpenoids (6.12±0.03 mg/g), flavonoids (9.54±0.05 mg/g), tannins (3.23±0.04 mg/g), and saponins (3.68±0.09 mg/g). The following amounts of vitamins and minerals found in the leaves extract; vitamin A (9.80±0.02 mg/100 g), vitamin C (12.42±0.07 mg/100 g), and vitamin E (7.25±0.03 mg/100 g); minerals: Iron (7.28±0.025 mg/100 g), magnesium (97.58±0.03 mg/100 g), and selenium (1.60±0.025 mg/100). The results obtained revealed that the plant Piper nigrum is endowed with free radical scavenging molecules and it can be used as a potential source of natural antioxidants in oxidative stressed conditions.