Mode of AmyR binding to the CGGN8AGG sequence in the Aspergillus oryzae taaG2 promoter
T Ito, S Tani, T Itoh, N Tsukagoshi, M Kato… - Bioscience …, 2004 - jstage.jst.go.jp
T Ito, S Tani, T Itoh, N Tsukagoshi, M Kato, T Kobayashi
Bioscience, biotechnology, and biochemistry, 2004•jstage.jst.go.jpAmyR is a transcriptional activator in Aspergillus spp. necessary for induction of the
amylolytic enzyme genes. It recognizes 50-CGGN8CGG-30 conserved in a number of the
amylolytic gene promoters, and in addition 50-CGGAAATTTAA-30 in the A. oryzaeamylase
promoter. In this report, interaction of AmyR with the 50-CGGAAATTTAA-30 type binding site
in the Taka-amylase gene (taaG2) promoter was precisely characterized by DNase I
footprinting analysis and electrophoretic mobility shift assay in vitro, and also by examination …
amylolytic enzyme genes. It recognizes 50-CGGN8CGG-30 conserved in a number of the
amylolytic gene promoters, and in addition 50-CGGAAATTTAA-30 in the A. oryzaeamylase
promoter. In this report, interaction of AmyR with the 50-CGGAAATTTAA-30 type binding site
in the Taka-amylase gene (taaG2) promoter was precisely characterized by DNase I
footprinting analysis and electrophoretic mobility shift assay in vitro, and also by examination …
AmyR is a transcriptional activator in Aspergillus spp. necessary for induction of the amylolytic enzyme genes. It recognizes 50-CGGN8CGG-30 conserved in a number of the amylolytic gene promoters, and in addition 50-CGGAAATTTAA-30 in the A. oryzaeamylase promoter. In this report, interaction of AmyR with the 50-CGGAAATTTAA-30 type binding site in the Taka-amylase gene (taaG2) promoter was precisely characterized by DNase I footprinting analysis and electrophoretic mobility shift assay in vitro, and also by examination of the in vivo activity of the mutated promoters. The in vitro and in vivo analyses indicated that two AmyR molecules bind cooperatively to the 50-CGGAAATTTAA-30 sequence by recognizing the CGG triplet at the 50-end and the AGG triplet just downstream of the sequence.
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