Performance evaluation and clinical associations of immunoassays that detect antibodies to negatively charged phospholipids other than cardiolipin
American journal of clinical pathology, 2018•academic.oup.com
Objectives We evaluate the performance characteristics of antiphosphatidylserine (anti-PS),
antiphosphatidylinositol (anti-PI), and antiphospholipid mixture (APhL) enzyme-linked
immunosorbent assays (ELISAs) compared with anticardiolipin (aCL) and anti–β2
glycoprotein I (anti-β2GPI) in a large group of patients with antiphospholipid (aPL)–related
diseases. Methods Serum samples from 548 patients from the Hopkins and Jamaican
systemic lupus erythematosus cohorts, the PROMISSE cohort, and the Antiphospholipid …
antiphosphatidylinositol (anti-PI), and antiphospholipid mixture (APhL) enzyme-linked
immunosorbent assays (ELISAs) compared with anticardiolipin (aCL) and anti–β2
glycoprotein I (anti-β2GPI) in a large group of patients with antiphospholipid (aPL)–related
diseases. Methods Serum samples from 548 patients from the Hopkins and Jamaican
systemic lupus erythematosus cohorts, the PROMISSE cohort, and the Antiphospholipid …
Objectives
We evaluate the performance characteristics of antiphosphatidylserine (anti-PS), antiphosphatidylinositol (anti-PI), and antiphospholipid mixture (APhL) enzyme-linked immunosorbent assays (ELISAs) compared with anticardiolipin (aCL) and anti–β2 glycoprotein I (anti-β2GPI) in a large group of patients with antiphospholipid (aPL)–related diseases.
Methods
Serum samples from 548 patients from the Hopkins and Jamaican systemic lupus erythematosus cohorts, the PROMISSE cohort, and the Antiphospholipid Standardization Laboratory were examined for immunoglobulin G (IgG)/immunoglobulin M (IgM) positivity in aCL, anti-β2GPI, anti-PS, anti-PI, and APhL ELISA assays.
Results
All IgG assays were associated with one or more thrombotic and/or obstetric manifestations, with an increased risk associated with higher antibody titers. Analytical performance was similar among assays, but IgG assays performed better than IgM counterparts.
Conclusions
Increasing titers of APhL, anti-PS, and anti-PI antibodies could indicate an increased risk of thrombotic and/or obstetric aPL-related manifestations. These assays may be promising biomarkers for particular APS manifestations.
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