Rapid detection of avian influenza virus a and subtype H5N1 by single step multiplex reverse transcription-polymerase chain reaction

HL Wei, GR Bai, AS Mweene, YC Zhou, YL Cong, J Pu… - Virus genes, 2006 - Springer
Outbreaks of H5N1 highly pathogenic avian influenza (HPAI) virus caused great economic
losses to the poultry industry and resulted in human deaths in Thailand and Viet Nam in
2004. Rapid typing and subtyping of H5N1 viruses, especially from clinical specimens, are
desirable for taking prompt control measures to prevent the spread of the disease. Here, we
developed a set of oligonucleotide primers able to detect, type and subtype H5 and N1
influenza viruses in a single step multiplex reverse transcription-polymerase chain reaction …

Rapid detection of avian influenza virus A and subtype H5N1 by single step multiplex reverse transcription-polymerase chain reaction.

WHL Wei HuiLing, BGR Bai GuiRong, AS Mweene… - 2006 - cabidigitallibrary.org
Outbreaks of H5N1 highly pathogenic avian influenza (HPAI) virus caused great economic
losses to the poultry industry and resulted in human deaths in Thailand and Viet Nam in
2004. Rapid typing and subtyping of H5N1 viruses, especially from clinical specimens, are
desirable for taking prompt control measures to prevent the spread of the disease. Here, we
developed a set of oligonucleotide primers able to detect, type and subtype H5 and N1
influenza viruses in a single step multiplex reverse transcription-polymerase chain reaction …
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