Rapid typing of truffle mycorrhizal roots by PCR amplification of the ribosomal DNA spacers
A Mello, C Nosenzo, F Meotto, P Bonfante - Mycorrhiza, 1996 - Springer
A Mello, C Nosenzo, F Meotto, P Bonfante
Mycorrhiza, 1996•SpringerDNA analyses were developed to type mycorrhizas of two Tuber species of commercial
value (T. melanosporum, T. borchii) and a competitive fungus (Sphaerosporella brunnea)
which forms ectomycorrhizas with plants usually considered hosts for truffles. Polymerase
chain reaction (PCR) amplification of DNA isolated from fruitbodies, mycelia, mycorrhizas
and leaves of host plants, was performed with a primer pair for an internal transcribed
spacer ITS1-4. ITS amplification followed by restriction fragment length polymorphism …
value (T. melanosporum, T. borchii) and a competitive fungus (Sphaerosporella brunnea)
which forms ectomycorrhizas with plants usually considered hosts for truffles. Polymerase
chain reaction (PCR) amplification of DNA isolated from fruitbodies, mycelia, mycorrhizas
and leaves of host plants, was performed with a primer pair for an internal transcribed
spacer ITS1-4. ITS amplification followed by restriction fragment length polymorphism …
Abstract
DNA analyses were developed to type mycorrhizas of two Tuber species of commercial value (T. melanosporum, T. borchii) and a competitive fungus (Sphaerosporella brunnea) which forms ectomycorrhizas with plants usually considered hosts for truffles. Polymerase chain reaction (PCR) amplification of DNA isolated from fruitbodies, mycelia, mycorrhizas and leaves of host plants, was performed with a primer pair for an internal transcribed spacer ITS1-4. ITS amplification followed by restriction fragment length polymorphism (RFLP) analysis of the amplified products clearly distinguished the two Tuber species at the fruitbody, mycorrhiza and mycelium levels.
Springer
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