Single-synapse ablation and long-term imaging in live C. elegans
Journal of neuroscience methods, 2008•Elsevier
Synapses are individually operated, computational units for neural communication. To
manipulate physically individual synapses in a living organism, we have developed a laser
ablation technique for removing single synapses in live neurons in C. elegans that operates
without apparent damage to the axon. As a complementary technique, we applied
microfluidic immobilization of C. elegans to facilitate long-term fluorescence imaging and
observation of neuronal development. With this technique, we directly demonstrated the …
manipulate physically individual synapses in a living organism, we have developed a laser
ablation technique for removing single synapses in live neurons in C. elegans that operates
without apparent damage to the axon. As a complementary technique, we applied
microfluidic immobilization of C. elegans to facilitate long-term fluorescence imaging and
observation of neuronal development. With this technique, we directly demonstrated the …
Synapses are individually operated, computational units for neural communication. To manipulate physically individual synapses in a living organism, we have developed a laser ablation technique for removing single synapses in live neurons in C. elegans that operates without apparent damage to the axon. As a complementary technique, we applied microfluidic immobilization of C. elegans to facilitate long-term fluorescence imaging and observation of neuronal development. With this technique, we directly demonstrated the existence of competition between developing synapses in the HSNL motor neuron.
Elsevier
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