Structural analysis and optimised production of fructo- oligosaccharides by levansucrase from Acetobacter diazotrophicus SRT4
Y Támbara, JV Hormaza, C Pérez, A León, J Arrieta… - Biotechnology …, 1999 - Springer
Y Támbara, JV Hormaza, C Pérez, A León, J Arrieta, L Hernández
Biotechnology Letters, 1999•SpringerMajor fructo-oligosaccharides (FOS) produced by levansucrase (EC 2.4. 1.10) from
Acetobacter diazotrophicus SRT4 were characterised as 1-kestose and nystose by acid
hydrolysis and 13C-NMR spectroscopy. The highest yields of 1-kestose (481 mM; 241 g/l)
and nystose (81 mM; 54 g/l) were achieved at initial sucrose concentration of 1754 mM (600
g/l), pH 5.5 and 40° C. The synthesized FOS reached 50%(w/w) of total sugars in the
reaction mixture, with a conversion efficiency over 70%(w/w) based on the amount of …
Acetobacter diazotrophicus SRT4 were characterised as 1-kestose and nystose by acid
hydrolysis and 13C-NMR spectroscopy. The highest yields of 1-kestose (481 mM; 241 g/l)
and nystose (81 mM; 54 g/l) were achieved at initial sucrose concentration of 1754 mM (600
g/l), pH 5.5 and 40° C. The synthesized FOS reached 50%(w/w) of total sugars in the
reaction mixture, with a conversion efficiency over 70%(w/w) based on the amount of …
Abstract
Major fructo-oligosaccharides (FOS) produced by levansucrase (EC 2.4.1.10) from Acetobacter diazotrophicus SRT4 were characterised as 1-kestose and nystose by acid hydrolysis and 13C-NMR spectroscopy. The highest yields of 1-kestose (481 mM; 241 g/l) and nystose (81 mM; 54 g/l) were achieved at initial sucrose concentration of 1754 mM (600 g/l), pH 5.5 and 40 °C. The synthesized FOS reached 50% (w/w) of total sugars in the reaction mixture, with a conversion efficiency over 70% (w/w) based on the amount of sucrose converted to 1-kestose.
Springer
以上显示的是最相近的搜索结果。 查看全部搜索结果