Substrate affinity differentially influences protein kinase C regulation and inhibitor potency
RF Sommese, S Sivaramakrishnan - Journal of Biological Chemistry, 2016 - ASBMB
The overlapping network of kinase-substrate interactions provides exquisite specificity in cell
signaling pathways, but also presents challenges to our ability to understand the
mechanistic basis of biological processes. Efforts to dissect kinase-substrate interactions
have been particularly limited by their inherently transient nature. Here, we use a library of
FRET sensors to monitor these transient complexes, specifically examining weak
interactions between the catalytic domain of protein kinase Cα and 14 substrate peptides …
signaling pathways, but also presents challenges to our ability to understand the
mechanistic basis of biological processes. Efforts to dissect kinase-substrate interactions
have been particularly limited by their inherently transient nature. Here, we use a library of
FRET sensors to monitor these transient complexes, specifically examining weak
interactions between the catalytic domain of protein kinase Cα and 14 substrate peptides …
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