Synthesis and characterization of ampelopsin glucosides using dextransucrase from Leuconostoc mesenteroides B-1299CB4: glucosylation enhancing …

HJ Woo, HK Kang, TTH Nguyen, GE Kim… - Enzyme and microbial …, 2012 - Elsevier
Novel ampelopsin glucosides (AMPLS-Gs) were enzymatically synthesized and purified
using a Sephadex LH-20 column. Each structure of the purified AMPLS-Gs was determined
by nuclear magnetic resonance, and the ionic product of AMPLS-G1 was observed at m/z
505 (C21H22O13· Na)+ using matrix-assisted laser desorption ionization time-of-flight mass
spectrometry. AMPLS-G1 was identified as ampelopsin-4′-O-α-d-glucopyranoside. The
optimum condition for AMPLS-G1, determined using response surface methodology, was …
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