Myenteric neurons of the guinea-pig ileum were intracellularly filled with the fluorescent dye
Lucifer Yellow, optically sectioned with a confocal microscope and volume reconstructed to
recreate 3-D images of the cells. The resulting images provide information not evident from
regular microscopy. The somata varied in cross-section from flat–oval to nearly circular, and
their surface membranes were marked by invaginations and protrusions significantly
increasing the surface area of the somatic membrane. The neurons could be divided into …