[PDF][PDF] Transforming p-coumaric acid into p-hydroxybenzoic acid by the mycelial culture of a white rot fungus Schizophyllum commune

A Sachan, S Ghosh, A Mitra - African Journal of Microbiology …, 2010 - academicjournals.org
African Journal of Microbiology Research, 2010academicjournals.org
Schizophyllum commune. TLC and HPLC chromatogram showed that p-hydroxybenzoic
acid was formed as a major degradation product of p-coumaric acid. The purity of p-
hydroxybenzoic acid was further confirmed by mass spectrometry. A maximum amount 2.5
mg/l of p-hydroxybenzoic acid was detected in the culture medium on 12th day of incubation
using 5.0 mM p-coumaric acid as sole source of carbon. It was assumed that during the
process of p-coumaric acid degradation, p-hydroxybenzaldehyde was produced as an …
Schizophyllum commune. TLC and HPLC chromatogram showed that p-hydroxybenzoic acid was formed as a major degradation product of p-coumaric acid. The purity of p-hydroxybenzoic acid was further confirmed by mass spectrometry. A maximum amount 2.5 mg/l of p-hydroxybenzoic acid was detected in the culture medium on 12th day of incubation using 5.0 mM p-coumaric acid as sole source of carbon. It was assumed that during the process of p-coumaric acid degradation, p-hydroxybenzaldehyde was produced as an unstable intermediate, which was rapidly converted into p-hydroxybenzoic acid and accumulated in the media. An indication of a very slow reverse conversion, that is, p-hydroxybenzoic acid into p-hydroxybenzaldehyde was also traced.
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