Abstract The Trier Social Stress Test (TSST) is a reliable and efficient protocol to induce
acute psychosocial stress in the laboratory. If circumstances do not allow in-person
assessments, an online version of the TSST could create more flexible research
opportunities. To date, studies have confirmed subjective and autonomic stress responses to
online TSST protocols. In this preregistered study (https://osf. io/u57aj), we focused on the
effect of a TSST online for adults (TSST-OA) on cortisol and alpha amylase levels, and …

Background The Trier Social Stress Test (TSST) is a reliable laboratory protocol to induce
acute psychosocial stress in the laboratory. If circumstances do not allow in-person
assessments, an online version of the TSST could create more flexible research
opportunities. To date, studies have confirmed subjective and autonomic stress responses to
online TSST protocols. In this preregistered study, we focused on the effect of a TSST online
for adults (TSST-OA) on cortisol and alpha amylase levels, and pleasure and arousal …