… sequencing technologies have revolutionized biology and biomedical sciences, sequencing
… RNA sequencing (RNA-Seq) enables transcript identification and quantification, but once …

… primer technology for automated sequencing has simplified methods for the direct sequencing
of … Another feature that is emerging from sequencing and concomitant functional analysis …

… This review emphasizes the particular contribution NGS-based technologies make to
functional genomics research with a special focus on gene regulation by transcription factor …

… Target sequencing was performed using the Haloplex custom target enrichment system (Agilent
Technologies, CA, USA) on the MiSeq sequencing platform with the MiSeq reagent kit …

… RNA sequencing to the most current microarray technology, RNA from the same samples
was analyzed on Affymetrix Mouse Gene 1.0 ST arrays at the Multiplexed Gene Analysis core …

… shuttle vector to facilitate functional gene studies. For genome mapping and sequencing, we
… useful resources for mouse genome mapping, sequencing projects, and functional analysis. …

… through faster sequencing will enable more powerful phylogenetic studies to be performed,
including those critical for human disease gene identification and functional analysis. …

The advent of high throughput next generation sequencing (NGS) has accelerated the pace
of discovery of disease-associated genetic variants and genomewide profiling of expressed …

… Here I survey next-generation sequencing technologies and consider how they can provide
… to characterize using next-generation sequencing technology. Here, the combination of …

… , genetic, and functional data. We suggest that a multidisciplinary pathway of assessment,
using several functional assays, will be required if next-generation sequencing is to be used …