Historically, genetic engineering in livestock proved to be challenging. Without stable
embryonic stem cell lines to utilize, somatic cell nuclear transfer (SCNT) had to be employed …

Traditionally, genetic engineering in the pig was a challenging task. Genetic engineering of
somatic cells followed by somatic cell nuclear transfer (SCNT) could produce genetically …

Genome editing tools (GET), including zinc-finger nucleases (ZFN), transcription activator-
like endonucleases (TALENS), and meganucleases possess long recognition sites and are …

Livestock is an essential life commodity in modern agriculture involving breeding and
maintenance. The farming practices have evolved mainly over the last century for …

Genome editing is a powerful technology that can efficiently alter the genome of organisms
to achieve targeted modification of endogenous genes and targeted integration of …

Contents In the last decade, the research community has witnessed a blooming of targeted
genome editing tools and applications. Novel programmable DNA nucleases such as zinc …

The production of transgenic farm animals (eg, cattle) via genome engineering for the gain
or loss of gene functions is an important undertaking. In the initial stages of genome …

After induced mutagenesis and transgenesis, genome editing is the next step in the
development of breeding techniques. Genome editing using site-directed nucleases …

Genetic modification of livestock has a longstanding and successful history, starting with
domestication several thousand years ago. Modern animal breeding strategies …

Pigs are an important resource in agriculture and serve as a model for human diseases. Due
to their physiological and anatomical similarities with humans, pigs can recapitulate …