Due to alternative splicing events in eukaryotic species, the identification of mRNA isoforms
(or splicing variants) is a difficult problem. Traditional experimental methods for this purpose …

Alternative splicing (AS) is a widespread process underlying the generation of transcriptomic
and proteomic diversity and is frequently misregulated in human disease. Accordingly, an …

To characterize the genetic variation of alternative splicing, we develop GLiMMPS, a robust
statistical method for detecting splicing quantitative trait loci (sQTLs) from RNA-seq data …

Many existing pipelines for scRNA-seq data apply pre-processing steps such as
normalization or imputation to account for excessive zeros or “drop-outs." Here, we …

Abstract Motivation Percent Spliced-In (PSI) values are commonly used to report alternative
pre-mRNA splicing (AS) changes. Previous PSI-detection tools were limited to specific AS …

High-throughput single-cell RNA-seq (scRNA-seq) is a powerful tool for studying gene
expression in single cells. Most current scRNA-seq bioinformatics tools focus on analysing …

RNA-seq experiments generate reads derived not only from mature RNA transcripts but also
from pre-mRNA. Here we present a computational approach called exon-intron split analysis …

We have mapped and quantified mouse transcriptomes by deeply sequencing them and
recording how frequently each gene is represented in the sequence sample (RNA-Seq) …

To the Editor—As single-cell RNA sequencing (scRNA-seq) becomes widespread,
accessible and scalable computational pipelines for data analysis are needed. We introduce …

The emerging single cell RNA sequencing (scRNA-seq) technologies enable the
investigation of transcriptomic landscapes at single-cell resolution. The analysis of scRNA …