Abstract We have established that 2, 3-diphosphoglycerate (2, 3-DPG) content and
intracellular pH exert separate, but interdependent, effects on the equilibrium solubility (csat) …

Although highly charged polyanions, such as inositol hexaphosphate, have been clearly
shown to decrease the solubility of deoxyhemoglobin S, the effect of 2, 3 …

We have examined the effect of 2, 3-diphosphoglycerate(DPG) on the solubility of deoxy-
sickle hemoglobin (deoxy-Hb S) under conditions such that concentration, pH, and …

Elevation of 2, 3-bisphosphoglycerate (2, 3-DPG) in sickle erythrocytes (SS RBCs) and
concomitant acidification of the cell interior promote polymerization by decreasing the …

The effects of 2, 3-diphosphoglycerate (DPG) and other allosteric polyanions of the
phosphate or sulfate ester class (inositol hexaphosphate (IHP), ATP, pyridoxamine-5' …

Recent work has enabled us to quantitate the four variables (2, 3-DPG concentration, pHi,
non-S hemoglobin composition, and O2 saturation) that modulate the equilibrium solubility …

Earlier observations indicated that volume exclusion by admixed non-hemoglobin
macromolecules lowered the polymer solubility (" C sat") of deoxyhemoglobin (Hb) S …

Ranjit Chatterjee, Roxanne Y. Walder, Arthur Arnone, and Joseph A. Walder*'1 abstract: In a
previous publication [Walder, JA, Walder, RY, & Arnone, A.(1980) J. Mol. Biol. 141, 195-216] …

A new turbidimetric method for the direct measurement of the solubility of oxy-and
deoxyhemoglobins (Hb) in concentrated phosphate buffer has been established. The …

The effects of varying pH and of increasing intracellular hemoglobin (Hb) concentration on
red cell sickling and oxygen affinity were studied in whole blood from persons with sickle cell …