Single-cell isoform RNA sequencing characterizes isoforms in thousands of cerebellar cells

I Gupta, PG Collier, B Haase, A Mahfouz… - Nature …, 2018 - nature.com
Full-length RNA sequencing (RNA-Seq) has been applied to bulk tissue, cell lines and
sorted cells to characterize transcriptomes,,,,,,,,,,, but applying this technology to single cells …

[HTML][HTML] Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq

V Hahaut, D Pavlinic, W Carbone, S Schuierer… - Nature …, 2022 - nature.com
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method
with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire …

Optimized single-nucleus transcriptional profiling by combinatorial indexing

BK Martin, C Qiu, E Nichols, M Phung… - Nature protocols, 2023 - nature.com
Single-cell combinatorial indexing RNA sequencing (sci-RNA-seq) is a powerful method for
recovering gene expression data from an exponentially scalable number of individual cells …

[HTML][HTML] Gene length and detection bias in single cell RNA sequencing protocols

B Phipson, L Zappia, A Oshlack - F1000Research, 2017 - ncbi.nlm.nih.gov
Background: Single cell RNA sequencing (scRNA-seq) has rapidly gained popularity for
profiling transcriptomes of hundreds to thousands of single cells. This technology has led to …

[HTML][HTML] An automated microwell platform for large-scale single cell RNA-seq

J Yuan, PA Sims - Scientific reports, 2016 - nature.com
Recent developments have enabled rapid, inexpensive RNA sequencing of thousands of
individual cells from a single specimen, raising the possibility of unbiased and …

Integrated genome and transcriptome sequencing of the same cell

SS Dey, L Kester, B Spanjaard, M Bienko… - Nature …, 2015 - nature.com
Single-cell genomics and single-cell transcriptomics have emerged as powerful tools to
study the biology of single cells at a genome-wide scale. However, a major challenge is to …

Single-cell sequencing

T Nawy - Nature methods, 2014 - nature.com
Single-cell sequencing | Nature Methods Skip to main content Thank you for visiting nature.com.
You are using a browser version with limited support for CSS. To obtain the best experience …

Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues

JH Lee, ER Daugharthy, J Scheiman, R Kalhor… - Nature protocols, 2015 - nature.com
Abstract RNA-sequencing (RNA-seq) measures the quantitative change in gene expression
over the whole transcriptome, but it lacks spatial context. In contrast, in situ hybridization …

Single-cell quantification of a broad RNA spectrum reveals unique noncoding patterns associated with cell types and states

A Isakova, N Neff, SR Quake - Proceedings of the National …, 2021 - National Acad Sciences
The ability to interrogate total RNA content of single cells would enable better mapping of
the transcriptional logic behind emerging cell types and states. However, current single-cell …

G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

IC Macaulay, W Haerty, P Kumar, YI Li, TX Hu… - Nature …, 2015 - nature.com
The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful
means to dissect genetic variation and its effect on gene expression. Here we describe G&T …