Single-cell isoform RNA sequencing characterizes isoforms in thousands of cerebellar cells
Full-length RNA sequencing (RNA-Seq) has been applied to bulk tissue, cell lines and
sorted cells to characterize transcriptomes,,,,,,,,,,, but applying this technology to single cells …
sorted cells to characterize transcriptomes,,,,,,,,,,, but applying this technology to single cells …
[HTML][HTML] Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq
V Hahaut, D Pavlinic, W Carbone, S Schuierer… - Nature …, 2022 - nature.com
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method
with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire …
with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire …
Optimized single-nucleus transcriptional profiling by combinatorial indexing
Single-cell combinatorial indexing RNA sequencing (sci-RNA-seq) is a powerful method for
recovering gene expression data from an exponentially scalable number of individual cells …
recovering gene expression data from an exponentially scalable number of individual cells …
[HTML][HTML] Gene length and detection bias in single cell RNA sequencing protocols
Background: Single cell RNA sequencing (scRNA-seq) has rapidly gained popularity for
profiling transcriptomes of hundreds to thousands of single cells. This technology has led to …
profiling transcriptomes of hundreds to thousands of single cells. This technology has led to …
[HTML][HTML] An automated microwell platform for large-scale single cell RNA-seq
Recent developments have enabled rapid, inexpensive RNA sequencing of thousands of
individual cells from a single specimen, raising the possibility of unbiased and …
individual cells from a single specimen, raising the possibility of unbiased and …
Integrated genome and transcriptome sequencing of the same cell
Single-cell genomics and single-cell transcriptomics have emerged as powerful tools to
study the biology of single cells at a genome-wide scale. However, a major challenge is to …
study the biology of single cells at a genome-wide scale. However, a major challenge is to …
Single-cell sequencing
T Nawy - Nature methods, 2014 - nature.com
Single-cell sequencing | Nature Methods Skip to main content Thank you for visiting nature.com.
You are using a browser version with limited support for CSS. To obtain the best experience …
You are using a browser version with limited support for CSS. To obtain the best experience …
Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues
Abstract RNA-sequencing (RNA-seq) measures the quantitative change in gene expression
over the whole transcriptome, but it lacks spatial context. In contrast, in situ hybridization …
over the whole transcriptome, but it lacks spatial context. In contrast, in situ hybridization …
Single-cell quantification of a broad RNA spectrum reveals unique noncoding patterns associated with cell types and states
The ability to interrogate total RNA content of single cells would enable better mapping of
the transcriptional logic behind emerging cell types and states. However, current single-cell …
the transcriptional logic behind emerging cell types and states. However, current single-cell …
G&T-seq: parallel sequencing of single-cell genomes and transcriptomes
The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful
means to dissect genetic variation and its effect on gene expression. Here we describe G&T …
means to dissect genetic variation and its effect on gene expression. Here we describe G&T …