[引用][C] Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics
TJ White - PCR Protocols: A guide to methods and applications …, 1990 - books.google.com
Comparative studies of the nucleotide sequences of ribosomal RNA (rRNA) genes provide a
means for analyzing phylogenetic relationships over a wide range of taxonomic levels …
means for analyzing phylogenetic relationships over a wide range of taxonomic levels …
Intragenomic variation of fungal ribosomal genes is higher than previously thought
Nuclear ribosomal genes in most eukaryotes are present in multiple copies and often used
for taxonomic and phylogenetic analyses. We comprehensively examined intragenomic …
for taxonomic and phylogenetic analyses. We comprehensively examined intragenomic …
A 5.8 S nuclear ribosomal RNA gene sequence database: applications to ecology and evolution
KW Cullings, DR Vogler - Molecular Ecology, 1998 - Wiley Online Library
We compiled a 5.8 S nuclear ribosomal gene sequence database for animals, plants, and
fungi using both newly generated and GenBank sequences. We demonstrate the utility of …
fungi using both newly generated and GenBank sequences. We demonstrate the utility of …
Sequencing and analysis of fungal rRNA operons for development of broad-range fungal PCR assays
PD Khot, DL Ko, DN Fredricks - Applied and environmental …, 2009 - Am Soc Microbiol
ABSTRACT rRNA genes are attractive targets for developing PCR assays targeting human
fungal pathogens. Most studies have focused on the 18S rRNA gene, internal transcribed …
fungal pathogens. Most studies have focused on the 18S rRNA gene, internal transcribed …
Detection of active soil fungi by RT-PCR amplification of precursor rRNA molecules
IC Anderson, PI Parkin - Journal of microbiological methods, 2007 - Elsevier
Microbial ecologists have used direct RT-PCR amplification of 16S rRNA molecules for the
detection of active species of bacteria and archaea, and 18S rRNA molecules for the …
detection of active species of bacteria and archaea, and 18S rRNA molecules for the …
Evolution of a large ribosomal RNA multigene family in filamentous fungi: birth and death of a concerted evolution paradigm
In eukaryotes, the primary components of the ribosome are encoded by multicopy nuclear
ribosomal RNA (rRNA) genes: 28/26S, 18S, 5.8 S, and 5S. Copies of these genes are …
ribosomal RNA (rRNA) genes: 28/26S, 18S, 5.8 S, and 5S. Copies of these genes are …
Evolutionary relationships within the fungi: analyses of nuclear small subunit rRNA sequences
Nucleotide sequences of the small subunit ribosomal RNA (18S) gene were used to
investigate evolutionary relationships within the Fungi. The inferred tree topologies are in …
investigate evolutionary relationships within the Fungi. The inferred tree topologies are in …
Organization of ribosomal RNA genes in the fungus Cochliobolus heterostrophus
The genes encoding the 17S, 5.8 S and 25S ribosomal RNAs in the Ascomycete
Cochliobolus heterostrophus were cloned and analyzed. They are arranged in tandemly …
Cochliobolus heterostrophus were cloned and analyzed. They are arranged in tandemly …
Concerted evolution in the ribosomal RNA cistron
Background Gene conversion is the mechanism proposed to be responsible for the
homogenization of multigene families such as the nuclear ribosomal gene clusters. This …
homogenization of multigene families such as the nuclear ribosomal gene clusters. This …
A nomenclature for fungal PCR primers with examples from intron-containing SSU rDNA
A Gargas, PT DePriest - Mycologia, 1996 - Taylor & Francis
We present a compilation of polymerase chain reaction primers (oligonucleotides) used to
amplify and sequence the small subunit of nuclear ribosomal DNA. To simplify use of these …
amplify and sequence the small subunit of nuclear ribosomal DNA. To simplify use of these …