Glycobiology and related disciplines have received an enormous interest in recent years as
they shed new light on the functional roles of carbohydrates in biological events leading to …

The mutation of putative acid/base and nucleophile of the active sites of retaining glycosyl
hydrolases, together with kinetic analysis of the mutants, and stereochemical identification of …

Remarkable advances in glycobiology in recent years have stimulated a resurgence of
interest in carbohydrate chemistry. The challenge of producing the complex glycosides and …

I. INTRODUCTION Recent demonstrations that oligosaccharides play important roles in
diverse biological events have resulted in renewed interest in the synthesis of …

Enzymes are indispensable tools in modern organic synthesis. Recent progress in cloning
techniques, microbiology and protein purification supply an ever growing number and …

Mammalian cell surfaces are all covered with bioactive oligosaccharides which play an
important role in molecular recognition events such as immune recognition, cell–cell …

Glycosynthases, engineered glycoside hydrolases that are able to synthesize glycans in
quantitative yields without hydrolyzing them, are among the most interesting tools for the …

In oligosaccharide synthesis the chemo‐enzymatic approach is often chosen to obtain the
desired glycosidic bond by exploiting the exquisite substrate specificity and stereoselectivity …

An overview of the applications of engineered glycosynthases and thioglycoligases for the
enzymatic synthesis of O-and S-glycosidic linkages in oligosaccharides is presented …

Retaining glycosidases have been extensively studied for the synthesis of oligosaccharides
and glycoconjugates as a convenient enzymatic approach to achieve the required regio-and …