The steadily increasing availability of human embryonic stem (hES) cell lines has created
strong interest in applying available tools for gene transfer in murine cells to human systems …

Oncoretroviral vectors have been successfully used in gene therapy trials, yet low
transduction rates and loss of transgene expression are still major obstacles for their …

Genetic modification of human embryonic stem cells (hESCs) is an important tool for
understanding and influencing their biologic properties. At the present time, lentiviral vectors …

The introduction of foreign genes into early mouse embryos and embryonic stem (ES) cells
is invaluable for the analysis of gene function and regulation in the living animal. The use of …

Here we describe the sustained expression of transgenes introduced into human embryonic
stem (ES) cells using self-inactivating lentiviral vectors. At low multiplicity of infection …

This chapter describes the methods we use to transduce mouse and human hematopoietic
stem cells (HSCs) and human embryonic stem cells (hESCs). We provide detailed protocols …

A variety of genetic and acquired diseases could conceivably be treated by gene therapy
targeted to hematopoietic stem cells (HSC). Inevitably, the effort to develop reliable methods …

Genetic modification of cells using retroviral vectors is the method of choice when the cell
population is difficult to transfect and/or requires persistent transgene expression in progeny …

The major limitations in gene transduction to embryonic stem (ES) cells are (1) low efficiency
of gene delivery and (2) suppression of gene expression after integration into the host …

The use of retroviral vectors for gene transfer into animals has been severely hampered by
the lack of provirus transcription in the early embryo and embryonic stem (ES) cells. This …