Harnessing CRISPR–Cas systems for bacterial genome editing
K Selle, R Barrangou - Trends in microbiology, 2015 - cell.com
Manipulation of genomic sequences facilitates the identification and characterization of key
genetic determinants in the investigation of biological processes. Genome editing via …
genetic determinants in the investigation of biological processes. Genome editing via …
Genome editing in bacteria: CRISPR-Cas and beyond
RD Arroyo-Olarte, R Bravo Rodriguez, E Morales-Ríos - Microorganisms, 2021 - mdpi.com
Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …
Bacterial Genome Editing with CRISPR-Cas9: Deletion, Integration, Single Nucleotide Modification, and Desirable “Clean” Mutant Selection in Clostridium beijerinckii …
CRISPR-Cas9 has been demonstrated as a transformative genome engineering tool for
many eukaryotic organisms; however, its utilization in bacteria remains limited and …
many eukaryotic organisms; however, its utilization in bacteria remains limited and …
The bacterial origins of the CRISPR genome-editing revolution
EJ Sontheimer, R Barrangou - Human gene therapy, 2015 - liebertpub.com
Like most of the tools that enable modern life science research, the recent genome-editing
revolution has its biological roots in the world of bacteria and archaea. C lustered, r egularly …
revolution has its biological roots in the world of bacteria and archaea. C lustered, r egularly …
Current and future prospects for CRISPR‐based tools in bacteria
ML Luo, RT Leenay, CL Beisel - Biotechnology and …, 2016 - Wiley Online Library
ABSTRACT CRISPR‐Cas systems have rapidly transitioned from intriguing prokaryotic
defense systems to powerful and versatile biomolecular tools. This article reviews how these …
defense systems to powerful and versatile biomolecular tools. This article reviews how these …
Barriers to genome editing with CRISPR in bacteria
Genome editing is essential for probing genotype–phenotype relationships and for
enhancing chemical production and phenotypic robustness in industrial bacteria. Currently …
enhancing chemical production and phenotypic robustness in industrial bacteria. Currently …
Evolution of CRISPR/cas systems for precise genome editing
M Hryhorowicz, D Lipiński, J Zeyland - International Journal of Molecular …, 2023 - mdpi.com
The bacteria-derived CRISPR/Cas (an acronym for regularly interspaced short palindromic
repeats/CRISPR-associated protein) system is currently the most widely used, versatile, and …
repeats/CRISPR-associated protein) system is currently the most widely used, versatile, and …
Endogenous CRISPR-Cas system-based genome editing and antimicrobials: review and prospects
Y Li, N Peng - Frontiers in microbiology, 2019 - frontiersin.org
CRISPR-Cas systems adapt “memories” via spacers from viruses and plasmids to develop
adaptive immunity against mobile genetic elements. Mature CRISPR RNAs guide CRISPR …
adaptive immunity against mobile genetic elements. Mature CRISPR RNAs guide CRISPR …
Cas9-based tools for targeted genome editing and transcriptional control
Development of tools for targeted genome editing and regulation of gene expression has
significantly expanded our ability to elucidate the mechanisms of interesting biological …
significantly expanded our ability to elucidate the mechanisms of interesting biological …
Multiplex genome editing of microorganisms using CRISPR-Cas
B Adiego-Perez, P Randazzo, JM Daran… - FEMS microbiology …, 2019 - academic.oup.com
Microbial production of chemical compounds often requires highly engineered microbial cell
factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful …
factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful …