Application of CRISPR-Cas9 systems has revolutionized genome editing across all domains
of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in …

Highlights•Cas9 provides a powerful counter-selection tool for genome editing in
bacteria.•CRISPRi is a versatile method for repressing gene expression.•With further …

Site-specific eukaryotic genome editing with CRISPR (clustered regularly interspaced short
palindromic repeats)-Cas (CRISPR-associated) systems has quickly become a …

CRISPR base editing is a powerful method to engineer bacterial genomes. However, it
restricts editing to single-nucleotide substitutions. Here, to address this challenge, we adapt …

Throughout the decades of its history, the advances in bacteria-based bio-industries have
coincided with great leaps in strain engineering technologies. Recently unveiled clustered …

Bacterial genome editing commonly relies on chromosomal cleavage with Cas nucleases to
counter-select against unedited cells. However, editing normally requires efficient …

As Cas9-mediated cleavage requires both protospacer and protospacer adjacent motif
(PAM) sequences, it is impossible to employ the CRISPR/Cas9 system to directly edit …

The targeting of nucleases to specific DNA sequences facilitates genome editing. Recent
work demonstrated that the CRISPR-associated (Cas) nuclease Cas9 can be targeted to …

Since the emergence of programmable RNA‐guided nucleases based on clustered
regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein …

Here we use the clustered, regularly interspaced, short palindromic repeats (CRISPR)–
associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations …