Hydrophobic interactions between the secondary structures on the molecular surface reinforce the alkaline stability of serine protease
We employed random mutagenesis to determine the region of the initial unfolding of hyper-
alkaline-sensitive subtilisin, ALP I, that precedes the denaturation of the entire protein under …
alkaline-sensitive subtilisin, ALP I, that precedes the denaturation of the entire protein under …
Improvement in the alkaline stability of subtilisin using an efficient random mutagenesis and screening procedure
BC Cunningham, JA Wells - Protein Engineering, Design and …, 1987 - academic.oup.com
An efficient random mutagenesis procedure coupled to a replica plate screen facilitated the
isolation of mutant subtilisins from Bacillus amyloliquefaciens that had altered autolytic …
isolation of mutant subtilisins from Bacillus amyloliquefaciens that had altered autolytic …
The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments
Y Yamagata, H Maeda, T Nakajima… - European journal of …, 2002 - Wiley Online Library
It is scientifically and industrially important to clarify the stabilizing mechanism of proteases
in extraordinary environments. We used subtilisins ALP I and Sendai as models to study the …
in extraordinary environments. We used subtilisins ALP I and Sendai as models to study the …
Kinetic analysis of enhanced thermal stability of an alkaline protease with engineered twin disulfide bridges and calcium‐dependent stability
K Ikegaya, S Sugio, K Murakami… - Biotechnology and …, 2003 - Wiley Online Library
The thermal stability of a cysteine‐free alkaline protease (Alp) secreted by the eukaryote
Aspergillus oryzae was improved both by the introduction of engineered twin disulfide …
Aspergillus oryzae was improved both by the introduction of engineered twin disulfide …
Enhanced stability of subtilisin by three point mutations
LO Narhi, Y Stabinsky, M Levitt, L Miller… - Biotechnology and …, 1991 - Wiley Online Library
This study was undertaken to characterize the effect of three point mutations made on aprA‐
subtilisin on the stability of the protein to both heat‐and detergent‐induced denaturation …
subtilisin on the stability of the protein to both heat‐and detergent‐induced denaturation …
Directed evolution of a subtilisin with calcium-independent stability
SL Strausberg, PA Alexander, DT Gallagher… - Bio/technology, 1995 - nature.com
Extracellular proteases of the subtilisin-class depend upon calcium for stability. Calcium
binding stabilizes these proteins in natural extracellular environments, but is an Achilles' …
binding stabilizes these proteins in natural extracellular environments, but is an Achilles' …
Molecular and structural characterization of a surfactant-stable high-alkaline protease AprB with a novel structural feature unique to subtilisin family
A Deng, J Wu, G Zhang, T Wen - Biochimie, 2011 - Elsevier
High-alkaline proteases are of great importance because of their proteolytic activity and
stability under high-alkaline condition. We have previously isolated a new protease (AprB) …
stability under high-alkaline condition. We have previously isolated a new protease (AprB) …
Engineering a Stable Protease
P Bryan, M Rollence, J Wood, S Quill, S Dodd… - Biotechnology Research …, 1988 - Springer
We have used several approaches to engineer large increases in the stability of the Bacillus
serine protease, subtilisin. These include introducing disulfide cross-links, improving …
serine protease, subtilisin. These include introducing disulfide cross-links, improving …
Contribution of a Salt Bridge Triad to the Thermostability of a Highly Alkaline Protease from an Alkaliphilic Bacillus Strain
T Kobayashi, Y Kageyama, N Sumitomo… - World Journal of …, 2005 - Springer
Crystallographic analysis of the highly alkaline M-protease from an alkaliphilic Bacillus
strain shows the occurrence of a unique salt bridge triad Arg19–Glu271–Arg275 (in …
strain shows the occurrence of a unique salt bridge triad Arg19–Glu271–Arg275 (in …
Thermostable variants of subtilisin selected by temperature‐gradient gel electrophoresis
A Sättler, S Kanka, KH Maurer, D Riesner - Electrophoresis, 1996 - Wiley Online Library
Region‐specific random mutagenesis in the weak calcium binding site of subtilisin
Carlsberg and subsequent screening for variants with enhanced heat stability revealed two …
Carlsberg and subsequent screening for variants with enhanced heat stability revealed two …