Like most of the tools that enable modern life science research, the recent genome-editing
revolution has its biological roots in the world of bacteria and archaea. C lustered, r egularly …

CRISPR technology has opened a new era of genome interrogation and genome
engineering. Discovered in bacteria, where it protects against bacteriophage by cleaving …

The last decade has seen the fields of molecular biology and genetics transformed by the
development of CRISPR-based gene editing technologies. These technologies were …

Editing DNA in eukaryotic cells with CRISPR-based systems has revolutionized the genome
engineering field. Cas (CRISPR-associated) endonucleases are directed to a particular …

Just a few years ago, molecular biologists hoping to alter the genome of their favorite
organisms faced an arduous task and likely weeks of genetic tinkering. Today, those …

Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

CRISPR-based genome editing holds promise for genome engineering and other
applications in diverse organisms. Defining and improving the genome-wide and …

Manipulation of genomic sequences facilitates the identification and characterization of key
genetic determinants in the investigation of biological processes. Genome editing via …

The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of
advances in the life sciences that have grown from the ability to edit genomes within living …

Clustered regularly interspaced short palindromic repeat-CRISPR-associated protein
(CRISPR-Cas) systems, found in nature as microbial adaptive immune systems, have been …