An efficient gene-targeting system based on impairment of the nonhomologous end-joining
pathway and the orotidine monophosphate decarboxylase gene (pyrG) in Aspergillus flavus …

Background An available whole genome sequence for Aspergillus flavus provides the
opportunity to characterize factors involved in pathogenicity and to elucidate the regulatory …

Aspergillus flavus often invade many important corps and produce harmful aflatoxins both in
preharvest and during storage stages. The regulation mechanism of aflatoxin biosynthesis in …

The industrial fungus Aspergillus luchuensis is used to produce a distilled spirit in Okinawa
Island, Japan. Recently, the genome sequence of A. luchuensis RIB2604 (Aspergillus …

Background The possibility for efficient gene targeting for the controlled integration of DNA
constructs is an important tool in fungal genetics. Findings In this study, we report a new …

Abstract A 8.6-kb disruption cassette, referred to here as a pyrG-blaster and consisting of the
Aspergillus niger pyrG gene flanked by a direct repeat that encodes the neomycin …

Aims: To develop a multiplex reverse transciption‐polymerase chain reaction (RT‐PCR)
protocol to discriminate aflatoxin‐producing from aflatoxin‐nonproducing strains of …

Aflatoxins are extremely potent carcinogens produced by Aspergillus flavus and Aspergillus
parasiticus. Cloning of genes in the aflatoxin pathway provides a specific approach to …

The rapid accumulation of genomic sequences from a large number of eukaryotes, including
numerous filamentous fungi, has created a tremendous scientific potential, which can only …

Aims: To establish a system that greatly increases the gene‐targeting frequency in
Aspergillus parasiticus. Methods and Results: The ku70 gene, a gene of the …