Background Early single-cell RNA-seq (scRNA-seq) studies suggested that it was unusual
to see more than one isoform being produced from a gene in a single cell, even when …

Single-cell RNAseq and alternative splicing studies have recently become two of the most
prominent applications of RNAseq. However, the combination of both is still challenging …

The emergence of single-cell RNA-seq (scRNA-seq) technology has made it possible to
measure gene expression variations at cellular level. This breakthrough enables the …

Single-cell RNA-seq (scRNA-seq) provides a comprehensive measurement of stochasticity
in transcription, but the limitations of the technology have prevented its application to dissect …

Background In eukaryotes, alternative splicing often generates multiple splice variants from
a single gene. Here weexplore the use of RNA sequencing (RNA-Seq) datasets to address …

RNA splicing is an important driver of heterogeneity in single cells through the expression of
alternative transcripts and as a determinant of transcriptional kinetics. However, the intrinsic …

The vast majority of multi-exon genes in higher eukaryotes are alternatively spliced and
changes in alternative splicing (AS) can impact gene function or cause disease. High …

Background In this paper, we address the problem of identifying and quantifying
polymorphisms in RNA-seq data when no reference genome is available, without …

Analysis of RNA sequencing (RNA-Seq) data have highlighted the fact that most genes
undergo alternative splicing (AS) and that these patterns are tightly regulated. Many of these …

Single cell RNA-seq experiments provide valuable insight into cellular heterogeneity but
suffer from low coverage, 3′ bias and technical noise. These unique properties of single …