Statistical design of crystallization experiments greatly reduces the amount of protein
necessary to find conditions for crystal growth and leads naturally to a useful data base for …

We have used the Incomplete Factorial Approach (Carter, CW, and Carter, CW, Jr.(1979) J.
Biol. Chem. 254, 12219-12223) in conjunction with the program Cristal (Roussel, A., Serre …

Protein crystal growth often depends on the combination of many different factors. Some
affect protein solubility directly; others may act indirectly by causing conformational changes …

A means of controlling crystallization is to separate the phases of nucleation and growth.
Methods to achieve this, other than seeding, involve lowering the supersaturation by …

In an effort to objectively compare the efficiency of protein crystallization screening
techniques, a probability model of sampling efficiency is developed and used to calculate …

In the crystallization of biomolecules two critical steps, the nucleation of the initial seed and
the enlargement of this seed, determine the quality of the final crystal. However, the degree …

MATERIALS AND METHODS Z'ryptophan tRNA Synthetase-All experiments used the
tryptophan specific aminoacyl tRNA synthetase from Bacillus stearothermophilus. This …

A simple device is described for controlling vapor equilibrium in macromolecular
crystallization as applied to the protein crystal growth technique commonly referred to as the …

Nucleation kinetics of tetragonal hen egg-white lysozyme obtained by the method of initial
rates are reported. Sodium chloride (NaCl), ranging in concentration from 2%(w/v) to …

[4] 77 methyl sulfoxide (DMSO) may be an example of this case, since it binds to the
hydrophobic groups of proteins, thereby ind Page 1 [4] NUCLEATION AND GROWTH 77 …