Contents Genome modification at specific loci in livestock species was only achievable by
performing homologous recombination in somatic cells followed by somatic cell nuclear …

Many genetically engineered rat strains have been produced for biomedical research. The
simple and quick production of knock-out and knock-in rats is currently possible using …

Genome editing is the process of making targeted modifications to the genome at site-
specific locations. The most common methods for genome editing are sequence-specific …

Traditionally, genetic engineering in the pig was a challenging task. Genetic engineering of
somatic cells followed by somatic cell nuclear transfer (SCNT) could produce genetically …

Gene editing techniques were developed chronologically, which include zinc finger
nuclease, transcription activator-like effector nuclease and clustered regularly interspaced …

Gene editing is a relatively recent concept in the molecular biology field. Traditional genetic
modifications in animals relied on a classical toolbox that, aside from some technical …

At the time of the first Transgenic Animal Research Conference, the lack of knowledge about
promoter, enhancer and coding regions of genes of interest greatly hampered our efforts to …

Great strides in technological advancements have been made in the past decade in cattle
genome engineering. First, the success of cloning cattle by somatic cell nuclear transfer …

In recent years, significant progress has been achieved in genome editing applications
using new programmable DNA nucleases such as zinc finger nucleases (ZFNs) …

Genetically-modified domestic animal models are of increasing significance in biomedical
research and agriculture. As authentic ES cells derived from domestic animals are not yet …