RNA splicing is an important driver of heterogeneity in single cells, both through the
expression of alternative transcripts and as a major determinant of transcriptional kinetics …

RNA splicing is an important driver of heterogeneity in single cells through the expression of
alternative transcripts and as a determinant of transcriptional kinetics. However, the intrinsic …

Alternative pre-mRNA splicing (AS) is a fundamental regulatory process that generates
transcript diversity and cell type variation. We developed Shiba, a robust method integrating …

The emergence of single-cell RNA-seq (scRNA-seq) technology has made it possible to
measure gene expression variations at cellular level. This breakthrough enables the …

Background: The advent of single-cell RNA sequencing (scRNA-seq) technology has
offered unprecedented opportunities to unravel cellular heterogeneity and functions. Yet …

Alternative splicing (AS) contributes significantly to RNA and protein variability yet its role in
defining cellular diversity is not fully understood. While Smart-seq2 offers enhanced …

Alternative splicing shapes the transcriptome and contributes to each cell's unique identity,
but single-cell RNA sequencing (scRNA-seq) has struggled to capture the impact of …

Motivation The RNA splicing efficiency is of high interest for both understanding the
regulatory machinery of gene expression and estimating the RNA velocity in single cells …

Alternative splicing is an important source of heterogeneity underlying gene expression
between individual cells but remains an understudied area due to the paucity of …

Single-cell RNA-seq (scRNA-seq) provides a comprehensive measurement of stochasticity
in transcription, but the limitations of the technology have prevented its application to dissect …