Genome editing is essential for probing genotype–phenotype relationships and for
enhancing chemical production and phenotypic robustness in industrial bacteria. Currently …

Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …

Microbial production of chemical compounds often requires highly engineered microbial cell
factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful …

As Cas9-mediated cleavage requires both protospacer and protospacer adjacent motif
(PAM) sequences, it is impossible to employ the CRISPR/Cas9 system to directly edit …

CRISPR-Cas9 has been demonstrated as a transformative genome engineering tool for
many eukaryotic organisms; however, its utilization in bacteria remains limited and …

Lactic‐acid bacteria such as Lactobacillus plantarum are commonly used for fermenting
foods and as probiotics, where increasingly sophisticated genome‐editing tools are …

Manipulation of genomic sequences facilitates the identification and characterization of key
genetic determinants in the investigation of biological processes. Genome editing via …

The clustered regularly interspaced short palindromic repeats (CRISPR)-associated
nuclease 9 (Cas9)-based genome editing tool pCas/pTargetF system that we established …

Highlights•Cas9 provides a powerful counter-selection tool for genome editing in
bacteria.•CRISPRi is a versatile method for repressing gene expression.•With further …

Genetic analysis is crucial to the understanding, exploitation, and control of microorganisms.
The advent of CRISPR‐Cas‐based genome‐editing techniques, particularly those mediated …