Aspergillus nidulans is an important experimental organism, and it is a model organism for
the genus Aspergillus that includes serious pathogens as well as commercially important …

The rapid accumulation of genomic sequences from a large number of eukaryotes, including
numerous filamentous fungi, has created a tremendous scientific potential, which can only …

Gene-targeting by homologous recombination occurs rarely during transformation since
nonhomologous recombination is predominant in Aspergillus oryzae. To develop a highly …

We have used a plasmid containing the argB gene to transform an Aspergillus nidulansargB-
deleted strain in the presence of restriction enzymes and show a 20-to 60-fold increase in …

In the koji molds Aspergillus sojae and Aspergillus oryzae, exogenous DNA is integrated in
the genome, in most cases irrespective of the sequence homology, suggesting that DNA …

An efficient gene-targeting system based on impairment of the nonhomologous end-joining
pathway and the orotidine monophosphate decarboxylase gene (pyrG) in Aspergillus flavus …

Mutants with a defective non-homologous-end-joining (NHEJ) pathway have boosted
functional genomics in filamentous fungi as they are very efficient recipient strains for gene …

We describe a rapid method for the production of fusion PCR products that can be used,
generally without band purification, to transform Aspergillus nidulans. This technique can be …

From an unstable Aspergillus nidulans colony, resulting from transformation with an A.
nidulans gene bank, a plasmid was reisolated which transformed A. nidulans at a frequency …

Gene targeting frequencies in Aspergillus niger are often very low and hamper efficient
functional genomics in this biotechnologically important fungus. Deletion of the A. niger kusA …