We have investigated transformation with heterologous DNA as a method for insertional
mutagenesis of Aspergillus fumigatus. Two methods, polyethylene glycol-mediated …

The advent of fluorescent proteins as vital dyes had a major impact in many research fields.
Different green fluorescent protein (GFP) variants were established in prokaryotic and …

In comparison with transformation vectors available for use in E. coli or yeast, there has
been relatively little development of vectors for use in filamentous fungi. For example …

When no phenotypic screen is available, gene disruption in the koji-mold Aspergillus sojae
is a time-consuming process, owing to the low frequency of homologous recombination. To …

A vector system was constructed that is designed to decrease the number of transformants
required to be screened when looking for gene disruption events in filamentous fungi. This …

Aspergillus oryzae is a safe mold widely used in food industry. It is also considered as a
microbial cell factory for production of recombinant proteins and enzymes. Currently, genetic …

Targeted gene replacement via homologous recombination is a routinely used approach to
elucidate the function of unknown genes. Integration of exogenous DNA in the genomic …

Background An available whole genome sequence for Aspergillus flavus provides the
opportunity to characterize factors involved in pathogenicity and to elucidate the regulatory …

Advances in genetic transformation techniques have made important contributions to
molecular genetics. Various molecular tools and strategies have been developed for …

Detailed evaluation of gene functions in an asexual fungus requires advanced methods of
molecular biology. For the generation of targeted gene deletions in the opportunistic …