In oligosaccharide synthesis the chemo‐enzymatic approach is often chosen to obtain the
desired glycosidic bond by exploiting the exquisite substrate specificity and stereoselectivity …

Glycosynthases, engineered glycoside hydrolases that are able to synthesize glycans in
quantitative yields without hydrolyzing them, are among the most interesting tools for the …

Oligosaccharide synthesis is becoming increasingly important to the pharmaceutical
industry because these biomolecules have potential application as therapeutics. Enzymatic …

The mutation of putative acid/base and nucleophile of the active sites of retaining glycosyl
hydrolases, together with kinetic analysis of the mutants, and stereochemical identification of …

Enzymes are indispensable tools in modern organic synthesis. Recent progress in cloning
techniques, microbiology and protein purification supply an ever growing number and …

Remarkable advances in glycobiology in recent years have stimulated a resurgence of
interest in carbohydrate chemistry. The challenge of producing the complex glycosides and …

The increasing recognition of the roles of carbohydrates in fundamental biological
processes and their potential as new therapeutics has accentuated the requirement for …

Thanks to the stability, good availability, stereoselectivity and broad substrate specificity,
oligosaccharide synthesis catalyzed by glycosidases represents an elegant way to complex …

Retaining glycosidases have been extensively studied for the synthesis of oligosaccharides
and glycoconjugates as a convenient enzymatic approach to achieve the required regio-and …

An overview of the applications of engineered glycosynthases and thioglycoligases for the
enzymatic synthesis of O-and S-glycosidic linkages in oligosaccharides is presented …