New approaches to enzymatic glycoside synthesis through directed evolution

R Kittl, SG Withers - Carbohydrate research, 2010 - Elsevier
The expanding field of glycobiology requires tools for the synthesis of structurally defined
oligosaccharides and glycoconjugates, while any potential therapeutic applications of sugar …

[HTML][HTML] Enzymatic synthesis of glycosides: from natural O-and N-glycosides to rare C-and S-glycosides

J Ati, P Lafite, R Daniellou - Beilstein journal of organic …, 2017 - beilstein-journals.org
Carbohydrate related enzymes, like glycosyltransferases and glycoside hydrolases, are
nowadays more easily accessible and are thought to represent powerful and greener …

Glycosynthases: mutant glycosidases for glycoside synthesis

SJ Williams, SG Withers - Australian journal of chemistry, 2002 - CSIRO Publishing
Glycosynthases are engineered mutant glycosidases that catalyse the formation of a
glycosidic bond from a glycosyl donor and an acceptor alcohol. They are constructed by …

Glycanase-catalyzed synthesis of non-natural oligosaccharides

S Shoda, M Fujita, S Kobayashi - Trends in Glycoscience and …, 1998 - jstage.jst.go.jp
A new methodology for the preparation of non-natural oligosaccharides has been
developed using various glycosyl fluorides as activated glycosyl donors for enzymatic …

Teaching old enzymes new tricks: engineering and evolution of glycosidases and glycosyl transferases for improved glycoside synthesis

FA Shaikh, SG Withers - Biochemistry and Cell Biology, 2008 - cdnsciencepub.com
The therapeutic potential of glycosides has made them an attractive target for drug
development. The biological extraction and chemical synthesis of these molecules is often …

Harnessing glycoenzyme engineering for synthesis of bioactive oligosaccharides

M Benkoulouche, R Fauré… - Journal of the …, 2019 - royalsocietypublishing.org
Combined with chemical synthesis, the use of glycoenzyme biocatalysts has shown great
synthetic potential over recent decades owing to their remarkable versatility in terms of …

Application of glycosidases and transglycosidases in the synthesis of oligosaccharides

FJ Plou, AG Segura, A Ballesteros - Industrial Enzymes: Structure …, 2007 - Springer
It is generally accepted that an oligosaccharide is a carbohydrate consisting of 2–10
monosaccharide residues linked by O-glycosidic bonds (Eggleston and Cote, 2003; …

Glycosidases—a great synthetic tool

M Scigelova, S Singh, DHG Crout - Journal of Molecular Catalysis B …, 1999 - Elsevier
Glycosidases were used to prepare oligosaccharide structures of physiological and
medicinal relevance. The study included an extensive screening of crude enzymatic …

[图书][B] Modern methods in carbohydrate synthesis

SH Khan - 2020 - books.google.com
Modern Methods in Carbohydrate Synthesis presents in one volume a sequence of chapters
leading from classical methods through to today's newest state-of-the-art technology for …

Recent developments in the use of enzymes in oligo-and polysaccharide synthesis

S Shoda, S Kobayashi - Trends in Polymer Science, 1997 - infona.pl
Oligo-and polysaccharides are important macromolecules in living systems, showing their
multifunctional characteristics in the construction of cell walls, energy storage, cell …