Detection of circulating neoplastic cells by reverse-transcriptase polymerase chain reaction in malignant melanoma: association with clinical stage and prognosis.

B Mellado, D Colomer, T Castel, M Munoz… - Journal of clinical …, 1996 - ascopubs.org
PURPOSE Circulating melanoma cells can be detected in peripheral blood by means of
tyrosinase mRNA amplification by reverse-transcriptase polymerase chain reaction (RT …

Prognostic significance of the detection of circulating malignant cells by reverse transcriptase-polymerase chain reaction in long-term clinically disease-free melanoma …

B Mellado, L Gutierrez, T Castel, D Colomer… - Clinical cancer …, 1999 - AACR
The purpose of this study was to assess the prognostic significance of the detection of
circulating melanoma cells by reverse transcriptase-PCR in long-term clinically disease-free …

Detection of circulating melanoma cells by specific amplification of tyrosinase complementary DNA is not a reliable tumor marker in melanoma patients: a clinical two …

R Gläser, K Rass, S Seiter, A Hauschild… - Journal of clinical …, 1997 - ascopubs.org
PURPOSE Recently, the reverse-transcription polymerase chain reaction (RT-PCR) of
tyrosinase messenger RNA (mRNA) was reported to be a useful tool for the detection of …

Reverse transcriptase-polymerase chain reaction for expression of tyrosinase to identify malignant melanoma cells in peripheral blood

K Pittman, S Burchill, J Southgate, J Joffe, M Gore… - Annals of oncology, 1996 - Elsevier
summary Background Circulating tumour cells in the peripheral blood may be important for
haematogenous spread of disease. The detection of these cells may therefore be a poor …

Polymerase chain reaction-based detection of circulating melanoma cells as an effective marker of tumor progression

G Palmieri, M Strazzullo, PA Ascierto… - Journal of clinical …, 1999 - ascopubs.org
PURPOSE: Reverse transcriptase (RT) polymerase chain reaction (PCR) with multiple
markers has been demonstrated to be highly sensitive in detecting circulating cells from …

Prognostic value of circulating melanoma cells detected by reverse transcriptase–polymerase chain reaction

G Palmieri, PA Ascierto, F Perrone… - Journal of clinical …, 2003 - ascopubs.org
Purpose: Factors that are predictive of prognosis in patients who are diagnosed with
malignant melanoma (MM) are widely awaited. Detection of circulating melanoma cells …

Prognostic role of circulating melanoma cells detected by reverse transcriptase-polymerase chain reaction for tyrosinase mRNA in patients with melanoma

C Visús, R Andres, JI Mayordomo… - Melanoma …, 2007 - journals.lww.com
A need for factors predictive of prognosis is present in patients who are diagnosed with
malignant melanoma. The detection of circulating melanoma cells by reverse transcriptase …

Detection of tumor-associated circulating mRNA in serum, plasma and blood cells from patients with disseminated malignant melanoma.

DO Hasselmann, G Rappl, M Rössler… - Oncology …, 2001 - spandidos-publications.com
Reverse transcription polymerase chain reaction (RT-PCR)-based detection of tyrosinase
mRNA is a frequently used method for the identification of circulating tumor cells in …

A polymerase chain reaction-based semiquantitative assessment of malignant melanoma cells in peripheral blood

P Brossart, JW Schmier, S Krüger, M Willhauck… - Cancer research, 1995 - AACR
Malignant melanoma cells can be detected with high sensitivity in peripheral blood of
patients using reverse transcription-PCR. The detection of tyrosinase mRNA that is actively …

The detection of circulating melanoma cells correlates with tumour thickness and ulceration but is not predictive of metastasis for patients with primary melanoma

GS Hanekom, HM Stubbings, CA Johnson… - Melanoma …, 1999 - journals.lww.com
We analysed peripheral blood samples from 143 patients with primary melanoma (PM) for
the presence of tyroslnase mRNA by reverse transcription-polymerase chain reaction (PCR) …