CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …

CRISPR base editing is a powerful method to engineer bacterial genomes. However, it
restricts editing to single-nucleotide substitutions. Here, to address this challenge, we adapt …

CRISPR-Cas9 has been demonstrated as a transformative genome engineering tool for
many eukaryotic organisms; however, its utilization in bacteria remains limited and …

Genome engineering is crucial for answering fundamental questions about, and exploring
practical applications of, microorganisms. Various microbial genome-engineering tools …

Here we use the clustered, regularly interspaced, short palindromic repeats (CRISPR)–
associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations …

Manipulation of genomic sequences facilitates the identification and characterization of key
genetic determinants in the investigation of biological processes. Genome editing via …

Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …

Microbial production of chemical compounds often requires highly engineered microbial cell
factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful …

CRISPR–Cas technologies have enabled programmable gene editing in eukaryotes and
prokaryotes. However, the leading Cas9 and Cas12a enzymes are limited in their ability to …

The ability to precisely edit individual bases of bacterial genomes would accelerate the
investigation of the function of genes. Here we utilized a nickase Cas9-cytidine deaminase …