Use of PCR to isolate genes encoding sigma54-dependent activators from diverse bacteria

RI Kaufman, BT Nixon - Journal of bacteriology, 1996 - Am Soc Microbiol
Degenerate PCR probes were used to amplify gene fragments encoding the catalytic
domain of sigma54-dependent transcription activators. The procedure should be widely …

Evidence of Multiple Regulatory Functions for the PtsN (IIANtr) Protein of Pseudomonas putida

I Cases, JA Lopez, JP Albar… - Journal of Bacteriology, 2001 - Am Soc Microbiol
The ptsN gene of Pseudomonas putida encodes IIANtr, a protein of the phosphoenol
pyruvate: sugar phosphotransferase (PTS) system which is required for the C source …

Modus operandi of the bacterial RNA polymerase containing the σ54 promoter‐specificity factor

S Wigneshweraraj, D Bose, PC Burrows… - Molecular …, 2008 - Wiley Online Library
Bacterial sigma (σ) factors confer gene specificity upon the RNA polymerase, the central
enzyme that catalyses gene transcription. The binding of the alternative σ factor σ54 confers …

Activation of a Cell Surface Signaling Pathway in Pseudomonas aeruginosa Requires ClpP Protease and New Sigma Factor Synthesis

TF Bishop, LW Martin, IL Lamont - Frontiers in microbiology, 2017 - frontiersin.org
Extracytoplasmic function (ECF) sigma factors control expression of large numbers of genes
in bacteria. Most ECF sigma factors are inhibited by antisigma proteins, with inhibition being …

sigma E changed to sigma B specificity by amino acid substitutions in its-10 binding region

KM Tatti, CP Moran Jr - Journal of bacteriology, 1995 - Am Soc Microbiol
The association of a sigma factor (sigma) with RNA polymerase in bacteria determines its
specificity of promoter utilization. To identify amino acid residues in sigma E from Bacillus …

The Role of Bacterial Enhancer Binding Proteins as Specialized Activators of σ54-Dependent Transcription

M Bush, R Dixon - Microbiology and Molecular Biology Reviews, 2012 - Am Soc Microbiol
Bacterial enhancer binding proteins (bEBPs) are transcriptional activators that assemble as
hexameric rings in their active forms and utilize ATP hydrolysis to remodel the conformation …

Identification and Functional Characterization of flgM, a Gene Encoding the Anti-Sigma 28 Factor in Pseudomonas aeruginosa

A Frisk, J Jyot, SK Arora, R Ramphal - Journal of bacteriology, 2002 - Am Soc Microbiol
We describe here the functional characterization of the putative flgM gene of Pseudomonas
aeruginosa. FlgM of P. aeruginosa is most similar to FlgM of Vibrio parahaemolyticus. A …

A full-length group 1 bacterial sigma factor adopts a compact structure incompatible with DNA binding

EC Schwartz, A Shekhtman, K Dutta, MR Pratt… - Chemistry & biology, 2008 - cell.com
The σ factors are the key regulators of bacterial transcription initiation. Through direct read-
out of promoter DNA sequence, they recruit the core RNA polymerase to sites of initiation …

Analysis of the rpoD gene encoding the principal sigma factor of Pseudomonas putida

M Fujita, Y Hanaura, A Amemura - Gene, 1995 - Elsevier
The gene (rpoD) encoding the principal α factor of Pseudomonas putida (Pp) was cloned
and sequenced. The amino-acid sequence deduced from the nucleotide sequence of rpoD …

Integrated activities of two alternative sigma factors coordinate iron acquisition and uptake by Pseudomonas aeruginosa

RJ Edgar, GE Hampton, GPC Garcia… - Molecular …, 2017 - Wiley Online Library
Alternative sigma (σ) factors govern expression of bacterial genes in response to diverse
environmental signals. In Pseudomonas aeruginosa σPvdS directs expression of genes for …