The CRISPR-Cas systems have shown tremendous promise as heterologous tools for
genome editing in various prokaryotes. However, the perturbation of DNA homeostasis and …

Genetic analysis is crucial to the understanding, exploitation, and control of microorganisms.
The advent of CRISPR‐Cas‐based genome‐editing techniques, particularly those mediated …

CRISPR/Cas9 is a versatile genome-editing tool which is widely used for modifying the
genome of both prokaryotic and eukaryotic organisms for basic research and applications …

Clustered regularly interspaced short palindromic repeats (CRISPR) is a family of DNA
direct repeats found in many prokaryotic genomes. It was discovered in bacteria as their …

Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …

The bacteria-derived CRISPR/Cas (an acronym for regularly interspaced short palindromic
repeats/CRISPR-associated protein) system is currently the most widely used, versatile, and …

Precise nucleic acid editing technologies have facilitated the research of cellular function
and the development of novel therapeutics, especially the current programmable nucleases …

CRISPR-Cas systems adapt “memories” via spacers from viruses and plasmids to develop
adaptive immunity against mobile genetic elements. Mature CRISPR RNAs guide CRISPR …

ABSTRACT CRISPR/Cas9 is an innovative molecular tool that is utilized in advanced
biological applications. This review focuses on modifying the genomes of a wide range of …

Clustered regularly interspaced short palindromic repeats (CRISPR) and its associated
enzymes (Cas) is a simple and convenient genome editing tool that has been used in …