Bottom-Up Fabrication of Protein Nanowires via Controlled Self-Assembly of Recombinant Geobacter Pilins

KM Cosert, A Castro-Forero, RJ Steidl, RM Worden… - MBio, 2019 - Am Soc Microbiol
Metal-reducing bacteria in the genus Geobacter use a complex protein apparatus to guide
the self-assembly of a divergent type IVa pilin peptide and synthesize conductive pilus …

An Escherichia coli Chassis for Production of Electrically Conductive Protein Nanowires

T Ueki, DJF Walker, TL Woodard, KP Nevin… - ACS synthetic …, 2020 - ACS Publications
Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires (e-PNs) are
a revolutionary electronic material. They offer novel options for electronic sensing …

Engineering Geobacter pili to produce metal: organic filaments

E Szmuc, DJF Walker, D Kireev, D Akinwande… - Biosensors and …, 2023 - Elsevier
The organized self-assembly of conductive biological structures holds promise for creating
new bioelectronic devices. In particular, Geobacter sulfurreducens type IVa pili have proven …

Voltammetric study of conductive planar assemblies of Geobacter nanowire pilins unmasks their ability to bind and mineralize divalent cobalt

KM Cosert, G Reguera - Journal of Industrial Microbiology and …, 2019 - academic.oup.com
Geobacter bacteria assemble a helical peptide of the Type IVa pilin subclass as conductive
pili decorated with metal binding and reduction sites. We used recombinant techniques to …

Harnessing the power of microbial nanowires

G Reguera - Microbial biotechnology, 2018 - Wiley Online Library
The reduction of iron oxide minerals and uranium in model metal reducers in the genus
Geobacter is mediated by conductive pili composed primarily of a structurally divergent pilin …

Biology and biotechnology of microbial pilus nanowires

MM Clark, G Reguera - … & Biotechnology: Official Journal of the …, 2020 - academic.oup.com
Abstract Type IV pili (T4P) are bacterial appendages used for cell adhesion and surface
motility. In metal-reducing bacteria in the genus Geobacter, they have the unique property of …

Microbial nanowires with increased conductivity and reduced diameters

DR Lovley, N Malvankar, R Adhikari, Y Tan… - US Patent …, 2021 - Google Patents
Improved electrically conductive pili were generated from a natural pilus protein from the
microorganism Geobacter sulfurreducens. Substituting a tryptophan for the phenylalanine at …

Electronic characterization of Geobacter sulfurreducens pilins in self-assembled monolayers unmasks tunnelling and hopping conduction pathways

KM Cosert, RJ Steidl, A Castro-Forero… - Physical Chemistry …, 2017 - pubs.rsc.org
The metal-reducing bacterium Geobacter sulfurreducens produces protein nanowires (pili)
for fast discharge of respiratory electrons to extracellular electron acceptors such as iron …

Intrinsically conductive microbial nanowires for 'green'electronics with novel functions

DR Lovley, J Yao - Trends in Biotechnology, 2021 - cell.com
Intrinsically conductive protein nanowires, microbially produced from inexpensive,
renewable feedstocks, are a sustainable alternative to traditional nanowire electronic …

Structural Basis for the Influence of A1, 5A, and W51W57 Mutations on the Conductivity of the Geobacter sulfurreducens Pili

C Shu, K Xiao, X Sun - Crystals, 2017 - mdpi.com
The metallic-like conductivity of the Geobacter sulfurreducens pilus and higher conductivity
of its mutants reflected that biological synthesis can be utilized to improve the properties of …