OBJECTIVE: In this study, we analyzed murine primitive erythropoiesis by coculturing Flk-1+
ES-derived cells with OP9 to find efficient culture conditions for erythroid cell induction. We …

OBJECTIVE: A variety of hematopoietic lineage cells have been produced from embryonic
stem (ES) cells, but their differentiation processes have not been elucidated well, especially …

OBJECTIVE: Erythropoietin (EPO) is the principal regulator for the production of adult-type
definitive erythrocytes (EryD). EPO not only stimulates both the proliferation and …

Primitive erythroid cells, the first red blood cells produced in the mammalian embryo, are
necessary for embryonic survival. Erythropoietin and its receptor EpoR, are absolutely …

We developed an efficient differentiation induction system from mouse embryonic stem (ES)
cells into blood cells by coculture on a novel stromal cell line named OP9, in order to …

An immortalized cell line representing the primitive erythroid (EryP) lineage was established
from in vitro–differentiated progeny (embryoid bodies [EBs]) of embryonic stem (ES) cells …

OBJECTIVE: During embryonic development murine erythropoiesis occurs in two waves by
producing first primitive erythroid cells (EryPs) and then definitive erythroid cells (EryDs) …

We reported previously that stimulation of glycoprotein 130 (gp130) by a combination of
human IL-6 and soluble IL-6 receptor (sIL-6R) could support proliferation, differentiation, and …

Our previous study demonstrated that transduction of murine embryonic stem (ES) cells with
a human erythropoietin (Epo) receptor (R) cDNA resulted in enhanced erythropoiesis in …

A critical issue for clinical utilization of human ES cells (hESCs) is whether they can
generate terminally mature progenies with normal function. We recently developed a …