Facultative intracellular pathogens are able to live inside and outside host cells. It is highly
desirable to differentiate their cellular locations for the purposes of fundamental research …

Pneumonia, a respiratory disease often caused by bacterial infection in the distal lung,
requires rapid and accurate identification, especially in settings such as critical care …

The quantitative understanding of cellular and molecular responses in living cells is
important for many reasons, including identifying potential molecular targets for treatments of …

Bacterial populations exhibit a range of metabolic states influenced by their environment,
intra-and interspecies interactions. The identification of bacterial metabolic states and …

In this review, we discuss multi-color single-molecule imaging and tracking strategies for
studying microbial cell biology. We first summarize and compare the methods in a detailed …

Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Significance: Fluorescence lifetime imaging microscopy (FLIM) measures the decay rate of
fluorophores, thus providing insights into molecular interactions. FLIM is a powerful …

Time-gated fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
assess the biochemistry of cells and tissues. When applied to living thick samples, it is …

To understand basic principles of living organisms one has to know many different
properties of all cellular components, their mutual interactions but also their amounts and …

Objectives: A novel Fluorescence Lifetime Imaging Microscopy (FLIM) deconvolution
method based on the linear expansion of fluorescence decays on a set of orthonormal …