Gene-targeting by homologous recombination occurs rarely during transformation since
nonhomologous recombination is predominant in Aspergillus oryzae. To develop a highly …

The industrial fungus Aspergillus luchuensis is used to produce a distilled spirit in Okinawa
Island, Japan. Recently, the genome sequence of A. luchuensis RIB2604 (Aspergillus …

Aspergillus nidulans is an important experimental organism, and it is a model organism for
the genus Aspergillus that includes serious pathogens as well as commercially important …

The construction of a fungal strain that lacks a specific gene product is often accomplished
by replacing the gene of interest with a selection marker using site-specific recombination …

The akuA gene encoding the Ku70 component of the nonhomologous end-joining
machinery was deleted in the opportunistic pathogen Aspergillus fumigatus. No obvious …

We have used a plasmid containing the argB gene to transform an Aspergillus nidulansargB-
deleted strain in the presence of restriction enzymes and show a 20-to 60-fold increase in …

The extent of published fungal genome sequences call for sophisticated genetic tools to
address the functions and features of annotated gene products. Gene targeting is the …

The rapid accumulation of genomic sequences from a large number of eukaryotes, including
numerous filamentous fungi, has created a tremendous scientific potential, which can only …

Previously we reported that double disruption of the proteinase genes (tppA and pepE)
improved heterologous protein production by Aspergillus oryzae (Jin et al. Appl Microbiol …

Marker rescue is an important molecular technique that enables sequential gene deletions.
The Cre-loxP recombination system has been used for marker gene rescue in various …