Bright ideas for chemical biology

LD Lavis, RT Raines - ACS chemical biology, 2008 - ACS Publications
Small-molecule fluorescent probes embody an essential facet of chemical biology. Although
numerous compounds are known, the ensemble of fluorescent probes is based on a modest …

Evolution of fluorescein as a platform for finely tunable fluorescence probes

Y Urano, M Kamiya, K Kanda, T Ueno… - Journal of the …, 2005 - ACS Publications
Fluorescence imaging is the most powerful technique currently available for continuous
observation of dynamic intracellular processes in living cells. Suitable fluorescence probes …

Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels

JB Grimm, LD Lavis - Nature Methods, 2022 - nature.com
The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed …

Improved chemical-genetic fluorescent markers for live cell microscopy

AG Tebo, FM Pimenta, Y Zhang, A Gautier - Biochemistry, 2018 - ACS Publications
Inducible chemical-genetic fluorescent markers are promising tools for live cell imaging
requiring high spatiotemporal resolution and low background fluorescence. The …

Bright photoactivatable fluorophores for single-molecule imaging

JB Grimm, BP English, H Choi, AK Muthusamy… - Nature …, 2016 - nature.com
Small-molecule fluorophores are important tools for advanced imaging experiments. We
previously reported a general method to improve small, cell-permeable fluorophores which …

Reaction-based small-molecule fluorescent probes for chemoselective bioimaging

J Chan, SC Dodani, CJ Chang - Nature chemistry, 2012 - nature.com
The dynamic chemical diversity of elements, ions and molecules that form the basis of life
offers both a challenge and an opportunity for study. Small-molecule fluorescent probes can …

Chronological development of functional fluorophores for bio-imaging

S Munan, YT Chang, A Samanta - Chemical Communications, 2023 - pubs.rsc.org
Functional fluorophores represent an emerging research field, distinguished by their diverse
applications, especially in sensing and cellular imaging. After the discovery of Quinine …

A general method to improve fluorophores for live-cell and single-molecule microscopy

JB Grimm, BP English, J Chen, JP Slaughter, Z Zhang… - Nature …, 2015 - nature.com
Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence
microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes …

Photoactivatable and photoconvertible fluorescent probes for protein labeling

D Maurel, S Banala, T Laroche… - ACS chemical biology, 2010 - ACS Publications
Photosensitive probes are powerful tools to study cellular processes with high temporal and
spatial resolution. However, most synthetic fluorophores suited for biomolecular imaging …

Miniaturized chemical tags for optical imaging

S Benson, F De Moliner, W Tipping… - Angewandte Chemie …, 2022 - Wiley Online Library
Recent advances in optical bioimaging have prompted the need for minimal chemical
reporters that can retain the molecular recognition properties and activity profiles of …