Principle has it that even the most advanced super‐resolution microscope would be futile in
providing biological insight into subcellular matrices without well‐designed fluorescent …

Conspectus Fluorescence microscopy and detection have become indispensible for
understanding organization and dynamics in biological systems. Novel fluorophores with …

Heptamethine indocyanines are invaluable probes for near-infrared (NIR) imaging. Despite
broad use, there are only a few synthetic methods to assemble these molecules, and each …

We have reengineered a red-emitting dicyanomethylenedihydrofuran push− pull
fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light …

Photoactivatable fluorophores are powerful imaging probes for tracking molecular and
cellular dynamics with high spatiotemporal resolution. We have designed and synthesized a …

Highlights•Protein–dye complexes that activate fluorescence allow no-wash protein
labeling.•Quencher removal, environmental change and direct binding can activate …

Fluorescence microscopy has revolutionised our understanding of biological systems,
enabling the visualisation of biomolecular structures and dynamics in complex systems. The …

Fluorophores have transformed the way we study biological systems, enabling non-invasive
studies in cells and intact organisms, which increase our understanding of complex …

The detection of chemical or biological analytes upon molecular reactions relies
increasingly on fluorescence methods, and there is a demand for more sensitive, more …

Fluorescence provides a mechanism for achieving contrast in biological imaging that
enables investigations of molecular structure, dynamics, and function at high spatial and …